Note: This figure is intended to serve only as a guide to those new to the ecoimmunology field. We expect that experts will disagree with our layout, and hope that they will suggest how and why to make alterations. If such comments are reasonable, changes will be made. Additions are welcome too, especially if they come with detailed protocols! In the end, we intend this figure to be a living one, much like Wikipedia. Where available, protocols are underlined and linked below.
Buffers and Solutions
Cell Counts and Cellular Activity
Total Haemocytes (invertebrate blood smears)
Total Leukocytes (vertebrate blood smears)
Lymphoid tissue mass
Circulating Immune Molecules (antibodies, cytokines, etc.)
Real-time PCR for cytokines
Antigen Dosing and Challenging
Fever and Sickness Behavior
Telemetry (radio and internal)
Swelling and Wound Repair
Antigen and parasite clearance
Plaque reduction neutralization test (PRNT)
cDNA synthesis from RNA (invitrogen: SuperScript III First-Strand Synthesis System)
Cloning kit (Invitrogen: TOPO TA Cloning Kit)
PCR Clean-up (Qiagen: QIAquick PCR Purification Kit)
Please use these protocols freely, but we request that if a publication results from their use, the authors acknowledge this website (www.ecoimmunology.org), the author(s) of the protocol used, and the grant that supports this site (NSF-0947177) in the acknowledgements sections. Also, please notify us at firstname.lastname@example.org of your publication so that we can list it on our Recent News page.
Please contact us (email@example.com) if you have protocols you would like to share on this website.